Kinetics Movie
This time series is from a calcium-flux experiment. In this experiment, peripheral T cells were stained with anti-CD markers to identify subsets and loaded with Indo-1. The ratio of Indo-1 for a gated subset of CD4 T cells is shown as a function of time. Approximately 30 seconds into the collection, anti-CD3 was added to stimulate the cells. The time in seconds since the beginning of the acquisition is shown in the upper left hand corner.
You will notice that about 230 seconds the first indications of calcium mobilization can be seen by the appearance of a peak of high Indo-1 ratio (Ca, X axis). Within 50 seconds after that a peak of high calcium concentration appears (at about 2 on the Ca scale), which quickly resolves into the main, intermediate peak (about 0.7 on the Ca scale), lasting to the end of the collection.
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