Compensation Example: Step 1
As a simple example, we will look at compensating a workspace which has five tubes collected: three compensation controls (one each for FITC, PE, and Cy5PE), and two PBMC samples that were stained with all three reagents. Our workspace shows these five samples, and the group that was created to hold this experiment:
For organization, we create a new group to hold just the compensation samples, and drag the three compensation samples into the group. Selecting this group results in only those samples being displayed as in the window below.
The next step is to define the gates for each of the singly-stained subpopulations.
Go back to the overview of compensation.