Getting Help in FlowJo
FlowJo is littered with help buttons throughout the application. Clicking on one of these launches a web browser to access our Web page describing that topic. Or, you can choose help from the Apple menu while running FlowJo to go directly to the general help page.
In addition, you can press the "help" key on your keyboard at any time. This will activate the help button on the frontmost window (if one exists); otherwise, it will provide you with general help on FlowJo.
Using the web browser, FlowJo gives you "context-sensitive help"--i.e., taking you to help information specifically regarding the operation you are currently performing.